INVESTIGADORES
OLAIZ Nahuel Manuel
congresos y reuniones científicas
Título:
Polymers for Biomedical and Pharmaceutical Applications
Autor/es:
MARSHALL GUILLERMO; OLAIZ NAHUEL; MOCSKOS ESTEBAN; COLOMBO LUCAS; SUAREZ CECILIA; GONZALEZ GRACIELA; RISK MARCELO; SOBA ALEJANDRO; NUÑEZ LUIS; CALVO JUAN CARLOS; MOLINA FERNANDO
Reunión:
Congreso; PPS-24 2008; 2008
Resumen:
In this study, tumor tissues
are simulated in vitro with natural polymer gels (collagens) to
elucidate electrochemical processes taking place during electrochemical
therapy. Use of electric currents in chemotherapy greatly enhances
drug transport and delivery synergistically with diffusion mechanism,
transport by migration, convection, or electroporation. Cancer
electrochemical treatment is based upon the passage of an electric
current, whether direct (electrochemical treatment, EChT) or micro
-pulsed (electrochemotherapy, ECT), through two or more electrodes
inserted locally in the tumor tissue. Extreme pH changes at tissue level
(EChT) with significant water motion or the creation of membrane
porous channels at the cell level (ECT), the presence of electric
fields facilitating transmembrane penetration of anticancer drugs into
the cell, tumor regression, are the main
electrochemical effects. Here we explore a combination of ECT and EChT
with drug-loaded nanoparticles with the final goal of enhancing drug
transport and delivery to tumor tissues and cells. Polymer measurements
are contrasted with in silico simulations (using a mathematical model
based on the Nernst-Planck, Poisson and Navier-Stokes equations for ion
transport, electric field distribution and fluid flow, respectively)
and with in vivo measurements with intravital microscopy
using BALB/c mice bearing a subcutaneous tumor under a dorsal window
chamber. Preliminary results suggest that the use of nanoparticle
charged drug delivery systems and tuned electric fields significantly
increases drug transport and delivery.