Antigenotoxic action of Minthostachys verticillata essential oil against damage induced by aflatoxin B1 in rats.

ESCOBAR FRANCO MATÍAS; SABINI MARÍA CAROLA; CARIDDI LAURA NOELIA; TORRES CRISTINA VANESA; PEREYRA CARINA; MAGNOLI ALEJANDRA; CAVAGLIERI LILIA

TAFI DEL VALLE

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

ASOCIACION DE BIOLOGIA DE TUCUMAN

ANTIGENOTOXIC ACTION OF Minthostachys verticillata ESSENTIAL OIL AGAINST DAMAGE INDUCED BY AFLATOXIN B1 IN RATSEscobar FM1; Sabini M1; Cariddi L1; Torres C1; Pereyra C1; Magnoli A1; Cavaglieri L11Dpto. Microbiología. UNRC, Ruta 8, Km 601 (5800), Río IV, Cbafescobar@exa.unrc.edu.arAflatoxin B1 (AFB1) is the most toxic mycotoxin and it is a common contaminant in foods. M. verticillata (peperina) is a plant that has a great ethnobotanical interest with multiple bioactivities demonstrated. The objective of this work was to determine antigenotoxic activity of M. verticillata essential oil (EO), against injury induced by AFB1 in rat bone marrow. Wistar rats were divided into four groups consisting of 5 rats in each group: group 1 common food (negative control); group 2 common food + EO (EO control); group 3 food with AFB1 [4 µg/kg] (toxin control); group 4 food with AFB1 + EO. The EO was dissolved in water at 0.04% (phospholipids emulsifying agents) to be administered in drinking. Food and water were available ad libitum throughout the experimental period (45 days). At the end of the study, the rats were decapitated and the femurs were immediately excised from the body. For each animal 2000 erythrocytes were counted to determine the frequency of micronuclei (MN). The average micronucleated erythrocytes (MNE) was 2.66? for the negative control group and 2.41? for the treated group with EO at 0.04%. In contrast, rats treated with AFB1 showed significant increase of MNE compared to other groups, with mean values of 4.67?*. The antigenotoxic effect of EO was observed in the group 4 (EO + AFB1). In this group, a decrease in the number of MN was observed compared to the group treated only with toxin. A similar average value of MNE compared to the negative control group was observed (3.01?). These results suggest that M. verticillata EO exerts antigenotoxic action against damage induced by AFB1 in hematopoietic bone marrow cells of rat.