PROTECTIVE EFFECT OF CHLOROGENIC ACID AGAINST GENOTOXIC DAMAGE INDUCED BY OCHRATOXIN A IN RATS

CAMPRA NOELIA; CARIDDI LAURA NOELIA; ESCOBAR FRANCO MATÍAS; SABINI MARÍA CAROLA; MAÑAS FERNANDO; SABINI LILIANA INÉS; DALCERO ANA MARIA

TAFI DEL VALLE

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

ASOCIACION DE BIOLOGIA DE TUCUMAN

PROTECTIVE EFFECT OF CHLOROGENIC ACID AGAINST GENOTOXIC DAMAGE INDUCED BY OCHRATOXIN A IN RATSCampra N1, Cariddi L1, Escobar F1, Sabini C1, Mañas F2, Sabini L1, Dalcero A1. 1Dpto. Microbiología e Inmunología (UNRC-CONICET), 2 Cátedra de Farmacología (UNRC). lcariddi@exa.unrc.edu.arOchratoxin A (OTA) is a mycotoxin with nephrotoxic, genotoxic and immunosuppressant properties. Some research indicates that the damage caused by OTA in the kidney may be associated with a potential carcinogen. In a previous study we confirmed that OTA induced DNA damage in bone marrow cells of mice and chlorogenic acid (ChlA) exerted a protective effect on this action but not through an antioxidant mechanism. The aim of this study was to characterize in vivo the ability to ChlA to reverse the genotoxic effects induced by OTA in a subacute toxicity test. Wistar rats were fed orally for 28 days with OTA (0.4 mg/kg), ChlA (5 mg/kg) or a combination of OTA (0.4 mg/kg)+ChlA(5 mg/kg). As vehicle control PBS/DMSO (0.03%) was used. The micronucleus assay on bone marrow and comet test on blood were performed. Increase of micronuclei (MN) in polychromatic erythrocytes (PCE) from OTA-treated animals was observed (p