ARACHIS HYPOGAEA L: CYTOTOXICITY STUDIES OF ETHANOLIC EXTRACTS AS ANTIVIRAL POTENTIAL

MENIS CANDELA FLORENCIA; SABINI MARÍA CAROLA; GIORDANO WALTER

TAFI DEL VALLE

Congreso; XXXIII Jornadas Científicas Asociación de Biología de Tucumán; 2016

ASOCIACION DE BIOLOGIA DE TUCUMAN

ARACHIS HYPOGAEA L: CYTOTOXICITY STUDIES OF ETHANOLIC EXTRACTS AS ANTIVIRAL POTENTIALMenis Candela F1, Sabini MC1, Giordano W2 1Dpto Mic e Inm, CsEFQyNat, UNRC. Ruta 36 Km 601. Río IV, Cba, Argentina. 2Dpto Biol Mol., UNRC. E-mail: csabini@exa.unrc.edu.arDengue virus (flia Flaviviridae) infects 50-100 million people each year worldwide. It causes different clinical symptoms: dengue fever, dengue hemorrhagic fever and dengue shock syndrome. Currently, there are no effective antiviral drugs for treating these illnesses. Peanut plant (Arachis hypogaea L) is a legume that has medicinal properties such as antioxidant, antiviral. The objective was to evaluate the cytotoxicity of A. hypogaea L extracts to determine safe concentrations for the host, to be tested against the dengue virus. Ethanolic extracts were obtained from seeds (SEE) and peanut skin (PSEE). Vero cell monolayers were treated with increasing concentrations (0-1600 µg/ml) of PSEE and SEE. Cytotoxicity was assessed at 2 and 7 days, by three assays: Maximum Non-Cytotoxic Concentration (MNCC), Neutral Red Uptake (NRU) and MTT reduction. MNCC values were 50 µg/ml for PSEE and 1000 µg/ml for SEE when cells were treated for 2 days. In the treatment for 7 days, MNCC were 10 and 300 µg/ml for PSEE and SEE, respectively. The CC50 values at 48 h were 600 .µg/ml (MTT) and >1600 µg/ml (NRU) for PSEE. While, SEE showed CC50>1400 µg/ml (MTT) and CC50=1600 µg/ml (NRU). Moreover, in treatment for 7 days it was determined for PSEE a CC50= 65 .µg/ml (MTT) and CC50>1400 µg/ml (NRU) and, for SEE CC50= 636 µg/ml (MTT) and CC50> 1400 µg/ml (NRU).In conclusion, it was possible to determine safe concentrations of extracts of A. hypogaea L which are useful to evaluate it as a potential anti-dengue. SEE showed very low toxicity against Vero cells. Conversely, PSEE showed greater toxicity.