Caenorhabditis elegans betaine-sensitive nicotinic receptors: Molecular function and physiological roles

TURANI, O.; HERNANDO, G.; RODRIGUEZ ARAUJO, N.; BOUZAT, C.

GLASGOW, SCOTLAND

Conferencia; 24th International C. elegans Conference; 2023

Genetics Society of America (GSA)

Caenorhabditis elegans possesses an extensive and diverse family of nicotinic receptors (nAChR), many of which remained uncharacterized. nAChRs are involved in worm locomotion and are targets of anthelmintic drugs. Parasitic nematodes have acquired resistance to most anthelmintic drugs, thus generating problems in human and animal health. Because of this, the identification of novel drugs and targets is required. The potent nematocidal drug monepantel (MNP), has been shown to target ACR-23 nAChR. ACR-23, whose endogenous agonist is betaine (BE), is a poorly characterized nAChR present in body-wall muscle and mechanosensory neurons of nematodes. Our goal is to decipher ACR-23 molecular function and its potential as a novel anthelmintic drug target. By performing locomotion assays with wild-type adult worms we showed that exogenous BE significantly increased worm motility. This effect was not observed in acr-23 mutants, indicating that the hypermotility is mediated by ACR-23. The exposure of worms to MNP produced the opposite effect, resulting in reduced motility as a function of concentration. MNP induced spastic paralysis and inhibited egg hatching, indicating important anthelmintic ability. Locomotion assays with mutant worms demonstrated that MNPinduced paralysis is mediated by ACR-23 and DEG-3/DES-2, a nAChR present in sensory neurons involved in nociception and chemotaxis. By patch-clamp recordings from cultured C. elegans L1 muscle cells, we described for the first time the properties of BE-elicited single-channel and macroscopic currents. Our study provides novel information aiming at the elucidation of the molecular function and pharmacology of the nAChR family. It also contributes to the understanding of the molecular basis of anthelmintic action, which paves the way for the development of novel drugs.