An ER-resident membrane protein complex regulates nicotinic acetylcholine receptor subunit composition at the synapse

RUTA B ALMEDOM; JANA F LIEWALD; GUILLERMINA HERNANDO; CHRISTIAN SCHULTHEIS; DIEGO RAYES; JIE PAN; THORSTEN SCHEDLETZKY; HARALD HUTTER; CECILIA BOUZAT; ALEXANDER GOTTSCHALK

EMBO JOURNAL

NATURE PUBLISHING GROUP

Lugar: Londres; Año: 2009 vol. 28 p. 2636 - 2649

0261-4189

Nicotinic acetylcholine receptors (nAChRs) mediate fast excitatory neurotransmission in neurons and muscles. To identify nAChR accessory proteins, which may regulate their expression or function, we performed tandem affinity purification of the levamisole-sensitive nAChR from Caenorhabditis elegans, mass spectrometry of associated components, and RNAi-based screening for effects on in vivo nicotine sensitivity. Among the proteins identified was the calcineurin A subunit TAX-6, which appeared to function as a negative regulator of nAChR activity. We also identified five proteins not previously linked to nAChR function, whose inactivation conferred nicotine resistance, implicating them as positive regulators of nAChR activity. Of these, the copine NRA-1 colocalized with the levamisole receptor at neuronal and muscle plasma membranes, and, when mutated, caused reduced synaptic nAChR expres-sion. Loss of SOC-1, which acts in receptor tyrosine kinase (RTK) signaling, also reduced synaptic levamisole recep-tor levels, as did mutations in the fibroblast growth factor receptor EGL-15, and another RTK, CAM-1. Thus, tándem affinity purification is a viable approach to identify novel proteins regulating neurotransmitter receptor activity or expression in model systems like C. elegans.