COMPARATIVE STUDY OF THE EFFECT OF NASAL AND ORAL Lactobacillus rhamnosus ADMINISTRATION ON INNATE IMMUNITY IN MALNOURISHED MICE

HERRERA MATÍAS; BARBIERI NATALIA; SALVA SUSANA; VILLENA JULIO; AGÜERO GRACIELA; ALVAREZ SUSANA

Tafí de Valle, Tucumán

Jornada; XXVII Jornadas Científicas de la Asociación de Biología de Tucumán; 2010

Asociación Argentina de Biología

The aim of this work was to study the effect of different routes of L. rhamnosus CRL1505 (Lr) administration on the improvement of the innate immune response (local and systemic) in malnourished mice. Different groups of malnourished mice were repleted with a balanced conventional diet (BD) for 7d, or BD for 7d with Lr by oral (OLr) or nasal route (NLr). At the end of each treatment, repleted groups, well-nourished (W) and malnourished (M) controls were challenged with Streptococcus pneumoniae (Sp). Before and after challenge, we studied: lung and blood infection, leukocyte counts in bronchoalveolar fluid (BAF), phagocytic activity in alveolar macrophages, total and differential cell counts and myeloperoxidase activity (Px+) in blood and bone marrow (BM). Before infection, NLr mice showed higher leukocyte and Px+ cell numbers than OLr group in BM. After challenge, NLr group had the lowest pathogen numbers in lung and the highest phagocytic activity in blood and BAF. In BM, both Lr treatments induced increased total cell numbers, mitotic pool cells and Px+ cells, but the NLr group showed higher values than OLr mice in these parameters. Hence, during a renutrition treatment, the nasal administration of Lr is more efficient than the oral way to improve the innate immunity against Sp respiratory infection.