An integrated physical map of the cultivated hot chili pepper Capsicum baccatum var. Pendulum (Solanaceae).

AGUILERA P.M.; DEBAT, H. J.; GRABIELE M.

Posadas

Jornada; Jornadas Cientifico Tecnologicas de la UNaM 2018; 2018

UNaM

Capsicum baccatum var. pendulum is the most frequently cultivated chili pepper in South America. This taxon is an outstanding source for breeding programs for genetic improvement in the genus since traits can be introgressed into C. annuum and related species from which viable hybrids are able to be generated.In this study, the chromosome complement of C. baccatum var. pendulum cv. ?cayenne? 2n=24 was deeply characterized through a combination of conventional and molecular cytogenetics approaches embracing heterochromatic fluorescence C-DAPI and CMA/DA/DAPI bandings and fluorescent in situ hybridization (FISH) of Capsicum-derived 5S and 18S-25S ribosomal genes and spacers probes. All the attained markers were integrated into a physical map which allows the identification of each individual chromosome in a single FISH assay. This is the first integrated physical map of cv. ?cayenne? which, in conjunction with reported genetic linkage maps of Capsicum, would be useful for future high-density genetic mapping and whole-genome studies in C. baccatum var. pendulum and related taxa as well as in breeding and genetic improvement programs in chili peppers. This study was funded by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT-Argentina), UNaM PICT 2014-3328 Préstamo BID Nº AR-L 1181.