Calcium intake affects body composition, bone metabolism and insulin resistance in spontaneously obese rats.

MAROTTE C; WEISSTAUB A; BRYK G; OLGUIN M; LUCERO DM; SCHREIER L; PORTELA ML; ZENI SN

San Diego, California

Congreso; 33rd Annual Meeting of the American Society for Bone and Mineral Research (ASBMR); 2011

American Society for Bone and Mineral Research (ASBMR)

MB Zemel theory postulated in 2004: “A high CaI would be a preventive factor for Ob/OW development”. Objective: to evaluate the effect of feeding different Ca content diet on body composition, bone metabolism and insulin-resistance in rats that spontaneously develop Ob in adolescent state. Female IIMbb rats fed from pregnancy one of 3 isocaloric diets only varied in Ca content: 0.9g% (HG), 0,5g% (NG) and 0,2g% (LG). At weaning male offspring continued feeding the same mother diet till 50 days of age (Tf). Results (mean ±SD), groups HG, NG & LG, respectively are shown in the table: HG NG LG Method Food intake 13.51±1.55 12.26±1.80 13.69±1.63 - Ca Intake (CaI) 221.0±15.0a 126.0±44.0b 46.0±9.0c - % Ca Absorption 64±6a 87±1b 91±9c [(ICa– Fecal)/ICa] x100. Body Weight (BW) (g) 195±9a 227±13b 262±7c - %Water 69.2±4.2 70.0±2.8 66.7±3.3 AOAC %Protein (g/100g BW) 14.3±1.7 14.2±1.9 15.9±2.1 AOAC %Fat 14.2±0.9a 13.7±0.9a 15.9±0.6b AOAC BMC (g/100g BW) 1.09±0.02a 1.29±0.08b 0.83±0.03c DXA P (mg/100g BW) 532±22a 520±31a 882±15b UV-Colorimetry BGP (ng/dl) 279±73a 375±46b 478±45c ELISA sCTX (mg/dl) 64.9±22.5a 71.9±12.4ab 80.6±14.1b ELISA Glucose (mg/dl) 111.8±25.5a 151.9±21.9b 207.0±11.0c Colorimetry Insulin (ng/ml) 1.92±0.75a 4.07±0.93b 6.87±2.24c ELISA Different letters: p