LuMPIS – A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein–protein interactions encoded by GC-low ORFs

MARIA GUADALUPE VIZOSO PINTO; JOSEFINA MARIA VILLEGAS; JAN PETER; RUDOLF HAASE; JURGEN HAAS; AMELIE SOPHIA LOTZ; ANIA CAROLINA MUNTAU; ARMIN BAIKER

PROTEOMICS (WEINHEIM. PRINT)

WILEY-V C H VERLAG GMBH

Año: 2009 vol. 9 p. 5303 - 5308

1615-9853

The GC content is highly variable among the genomes of different organisms. It has beenshown that recombinant gene expression in mammalian cells is much more efficient whenGC-rich coding sequences of a certain protein are used. In order to study protein–proteininteractions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novelluminescence-based maltose-binding protein pull-down interaction screening system(LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs inmammalian expression systems.