ISOLATION AND CHARACTERIZATION OF AN ABIETANE DITERPENE FROM COLEUS NEOCHILUS WITH POTENT ANTIPROLIFERATIVE ACTIVITY ON A HUMAN BREAST CANCER CELL LINE

CARLA LUCIANA MAYORA JUSTEL; TAMARA VALLADARES; VALERIA CAVALLARO; ANA PAULA MURRAY; ISABEL ALICIA LÜTHY; MARÍA DEL CARMEN ESANDI; ARIANA BRUZZONE; NATALIA PAOLA ALZA

Otro; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; 2023

Coleus spp. have diverse ethnobotanical applications, with their most prevalent use being attributed to their medicinal properties. We have previously described the antiproliferative effect of the ethanolic extract from C. neochilus (also known as "boldo rastrero") on different human breast cancer cell lines. The present study is focused on the extraction and isolation of compounds responsible for this effect. The ethanol extract (BRET) was subjected to solvent-solvent extraction, yielding four different sub-extracts: hexane (BRHX), chloroform (BRCL), ethyl acetate (BRAE), and methanol-water (BRAM). Remarkably, incubation with BRAE (50 μg/ml) was more effective in reducing the viability of human breast cancer cell line MCF-7 than the other fractions (BRET: 23%, BRHX: 29%, BRCL: 52%, BRAE: 68%, and BRAM: 10% reduction; p