Optimization of the Enzymatic Synthesis of Pentyl Oleate with Lipase Immobilized onto Novel Structured Support

CAVALLARO VALERIA; TONETTO, GABRIELA MARTA; FERREIRA, MARÍA LUJÁN

Fermentation

MDPI

Lugar: Basel; Año: 2019 vol. 5 p. 48 - 58

2311-5637

The term biorefinery is related to the sustainable production of value-added bioproductsand bioenergy from biomass. Esters from fatty acids are important compounds synthesized fromby-products of the oleochemical industry. In agreement with the biorefinery concept, it is importantto search for catalysts that reduce the consumption of energy and water, using moderate operationconditions and low reaction times. In this work, response surface methodology (RSM) was used tooptimize the enzymatic synthesis of pentyl oleate using Candida antarctica lipase B (CALB) immobilizedon a polyethylene-aluminum structured support. A factorial design was employed to evaluate theeects of several parameters on the ester yield. To obtain a model with a good fit, an approach toreaction mechanism and enzyme kinetics was taken into consideration. Experimental findings werecorrelated and explained using equations of a ping-pong bi-bi kinetic model and considering theinhibitory eects of both substrates. The developed model was consistent with the experimental datapredicting an increase in pentyl oleate production with increasing temperature and a decrease withhigher oleic acid amounts and alcohol to acid molar ratios. This model could be useful in a futureindustrial application of CALB/LLDPE/Al to minimize the costs in oleochemical biorefineries.