Claudin-4 expression in placentas from a murine model infected with Salmonella enterica

BETANCOURT DIANA; PUSTOVRH MC,; GAROFALO AILIN; GIACOMODONATO M; NOTO LLANA M

MACEIO

Congreso; X ENCUENTRO DE LA SOCIEDAD LATINOAMERICANA DE INTERACCIÓN MATENO-FETAL Y PLACENTA SLIMP 2024; 2024

SOCIEDAD LATINOAMERICANA DE INTERACCIÓN MATENO-FETAL Y PLACENTA

Objective. To evaluate the expression and immunolocalization of claudin-4 (CLDN4) in placentasfrom a murine model infected with Salmonella enterica.Methods. 8-week-old female CF1 mice received a high dose of Salmonella enterica (SE) (2x108CFU/mouse) intragastrically. After 20 days post-infection, females were mated with males of thesame strain. At gestational day 18 (day 38 post-infection) the pregnant females were sacrificedand 2-3 placentas per female were removed. The expression and localization of claudin-4(CLDN4) in control and infected placentas were determined by real-time PCR,immunohistochemical staining and immunofluorescence. All experimental protocols wereapproved by the Animal Ethics Committee, University of Buenos Aires.Results. In response to SE infection, the level of CLDN4 mRNA expression was significantly lowercompared to placentas from the control group (p