LACTOBACILLUS PLANTARUM ISOLATED FROM KEFIR: PROTECTION OF CULTURED HEP-2 CELLS AGAINST SHIGELLA INVASION

KAKISU E.; BOLLA, P. A.; ABRAHAM A.G; DE URRAZA P.J; DE ANTONI G.L

INTERNATIONAL DAIRY JOURNAL

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 p. 64 - 71

0958-6946

Two types of microbial mixtures containing selected lactic acid bacteria and yeast strains 30 isolated from kefir grains were tested against Shigella invasion on Hep-2 cells. A five-strain 31 mixture demonstrated an important protection against the internalization of Shigella flexneri 32 and Shigella sonnei. Preincubation of cells monolayer with mixtures or single kefir 33 microorganisms achieved a more efficient protection when it compared with the simultaneous 34 coincubation with Shigella. Among the microorganisms, Lactobacillus plantarum strain 35 CIDCA 83114 showed the most significant protection. Cell walls of L. plantarum as well as 36 the whole bacteria demonstrate an equivalent protection of epithelial monolayer; probably a 37 protein or peptide factor could be related with this effect. Our results constitute one of the first 38 steps to understand the potential properties of L. plantarum CIDCA 83114 isolated from kefir 39 against Shigella invasion and demonstrate that this strain is suitable to be part of potential 40 probiotic multistrain dairy starters.