HEME OXYGENASE 1 (HO-1) MODULATES RELEVANT METASTASIS-ASSOCIATED GENES IN PROSTATE CANCER

AGUSTINA SABATER; AYELEN TORO; PABLO SANCHIS; GASTON PASCUAL; ROCIO SENIUK; JAVIER COTIGNOLA; ELBA VAZQUEZ; GERALDINE GUERON; SOFIA LAGE VICKERS

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2022

Eighty percent of prostate cancers (PCa) metastasize to bone, showing a significant source of patient morbidity. PCa stem-like cells (PCSCs) are capable to outlast in a quiescent state remaining able to develop therapy-resistant tumors and metastatic lesions. We have previously reported that heme oxygenase 1 (HO-1, the rate limiting enzyme in heme degradation) overexpression leads to a less invasive PCa phenotype. However, its effect on metastasis-stemness (MS) remains unknown. In this work, we address the biological significance of HO-1 in association with MS genes relevant for PCa. Clonogenic assays were performed in PC3 and C4-2B cells subjected to hemin treatment (FDA approved drug and specific HO-1 inducer) to assess its effect on stem-like properties. Colony formation assays evidenced a reduction on stem-like properties of PCa cells treated with hemin (p