INVESTIGADORES
PETRIGH Romina Sandra
artículos
Título:
Molecular identification of Nematodirus spathiger (Nematoda: Molineidae) in Lama guanicoe from Patagonia Argentina
Autor/es:
ROMINA PETRIGH; MARTIN FUGASSA
Revista:
HELMINTHOLOGIA
Editorial:
VERSITA
Referencias:
Lugar: Varsovia; Año: 2014 vol. 51
ISSN:
0440-6605
Resumen:
The guanaco (Lama guanicoe) is the major inhabitant and
the largest wild artiodactyl in Patagonia. The introduction of
invasive species into its ecological niche poses ecological
risks, since invasive species may introduce harmful parasites
to this native species. In this work, filariform larvae of the
Nematodirus genus were found in feces of guanacos from
the Perito Moreno National Park in Argentina. All species
were characterized according to morphological features and
molecular analyses using ribosomal DNA (rDNA). For the
molecular analysis, rDNA fragments were amplified by
PCR and then sequenced. The results of the BLASTN
comparison threw a 99 % of identity with Nematodirus
spathiger and 97 % with N. helvetianus, suggesting that N.
spathiger is the infecting parasite. Nematodirus spathiger
together with N. filicollis and N. battus causes diarrhea and
deaths in sheep and, in some cases, in South American
camelids. The availability of more accurate diagnostic
methods such as PCR could improve the control measures
for gastrointestinal helminthiasis.