INVESTIGADORES
MACOR Lorena Paola
artículos
Título:
Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
Autor/es:
IBARRA, LUIS EXEQUIEL; PORCAL, GABRIELA VALERIA; MACOR, LORENA PAOLA; PONZIO, RODRIGO ANDRÉS; SPADA, RAMIRO MARTIN; LORENTE, CAROLINA; CHESTA, CARLOS ALBERTO; RIVAROLA, VIVIANA ALICIA; PALACIOS, RODRIGO EMILIANO
Revista:
NANOMEDICINE
Editorial:
FUTURE MEDICINE LTD
Referencias:
Lugar: Londres; Año: 2018 vol. 13 p. 605 - 624
ISSN:
1743-5889
Resumen:
Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-altbenzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNswith localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.