REDUCED EXCRETION OF Escherichia coli O157:H7 IN CATTLE AFTER SYSTEMIC VACCINATION WITH

VILTE DA; LARZÁBAL M; GARBACCIO S; GAMELLA M; RABINOVITZ B; ELIZONDO AM; DELGADO F; MEIKLE V; CATALDI A; MERCADO EC

Buenos Aires

Simposio; 7th International Symposium on Shiga Toxin (verocytotoxin)- producing Escherichi coli infections; 2009

          Ø  Enterohemorrhagic Escherichia coli O157: H7 was the most prevalent EHEC serotype recovered from patients with hemolytic uremic syndrome in Argentina, a country with a high incidence of this disease in children Ø  Vaccination of cattle, the main reservoir of EHEC O157: H7, could be a logical strategy to reduce the incidence of infection in humans Ø  This study evaluated a vaccine based in the carboxyl-terminal fragment of 280 amino acids of γ-intimin and EspB, two key colonization factors of E. coli O157: H7 ü  Intramuscular immunization of calves with Intimin and EspB  supplemented with an oiled adjuvant plus calcitriol resulted in both systemic and mucosal immune responses. ü   Vaccination significantly reduced the total bacteria shed in feces and the number of animals that shed of E. coli O157:H7 after ü   Systemic immunization of cattle with recombinants γ-Intimin-C280 and EspB proteins seems to be a feasible strategy to reduce EHEC O157:H7 fecal shedding.