INVESTIGADORES
PARACHU MARCO Maria Virginia
artículos
Título:
Characterization of Serum Complement Activity of Broad-Snouted Caiman (Caiman latirostris, Crocodylia: Alligatoridae)
Autor/es:
SIROSKI, PABLO; MERCHANT, MARK; PARACHÚ MARCÓ M.V.; PIÑA, C.I.; ORTEGA, HUGO
Revista:
ZOOLOGICAL STUDIES
Editorial:
Elsevier
Referencias:
Año: 2010 vol. 49 p. 64 - 70
ISSN:
1021-5506
Resumen:
The sheep red blood cell (SRBC) hemolysis assay was developed to detect complement system activity. Based on this technique, we detected and characterized the complement system activity of broad-snouted caiman (Caiman latirostris) serum. Hemolytic activity of caiman serum was inhibited by 2 classical inhibitors (EDTA and heat) suggesting that the ability of C. latirostris serum could be attributed to a complement system activity.We found that capacity of C. latirostris serum to disrupt SRBCs is concentration-, temperature-, and kinetics dependent. Hemolytic activity was detected at very low concentration (
