PERSONAL DE APOYO
YUNES QUARTINO Pablo Javier
congresos y reuniones científicas
Título:
A lectin and phospholipases A2 from Bothrops diporus venom: identification and sequencing by mass spectrometry
Autor/es:
PABLO J. YUNES QUARTINO; MADELÓN PORTELA; ANALÍA LIMA; ROSARIO DURÁN; GERARDO D. FIDELIO
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XLII Reunión Anual de la Sociedad Argentina de Biofísica; 2013
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
A lectin and phospholipases A2 from Bothrops diporus venom: identification and sequencing by mass spectrometryYunes Quartino, Pablo J.a, Portela, M.b, Lima A.b, Durán R.b and Fidelio G.D.aaCIQUIBIC, CONICET, Universidad Nacional de Córdoba, Argentina. bInstitut Pasteur de Montevideo, Uruguay.We report protein sequences from lipolitic fractions of venom of Bothrops diporus, a viper from Argentina known as Yarará Chica. We have previously cloned two phospholipases and produce them via heterologous expression and subsequent renaturation (1). By similarity and identity to public database sequences, we classified these as type A2 (PLA2). In the present work we used conventional chromatographic techniques coupled to MALDI-TOF-TOF mass spectrometry analysis to prove the existence at the protein level of the cloned sequences and portions of new PLA2 isospecies. Along this work we also found a lectin dimer for which it is proposed the disulfide intermolecular connectivity.AcknowledgementsCONICET, FONCYT, SECYT-UNC (Argentina) and Unidad de Proteómica y Bioquímica Analíticas, Institut Pasteur de Montevideo (Uruguay).1- Yunes Quartino P.J., Barra J.L., Fidelio G.D. Biochemical and Biophysical Research Communications.2012.321
