Simultaneous electroanalytical determination of luteolin and rutinusing artificial neural networks

A. Y. TESIO; S. N. ROBLEDO; A. M. GRANERO; H. FERNANDEZ; M. A. ZON

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Año: 2014 vol. 203 p. 655 - 662

0925-4005

In this study, we propose an electroanalytical method to quantify simultaneously luteolin and rutin,two flavonoids which are present in a pharmaceutical formulation. The methodology is based on squarewave voltammetry at glassy carbon electrodes modified with multiwalled carbon nanotubes dispersed inpolyethylenimine. Both flavonoids show quasi-reversible surface redox couples in 10% ethanol + 1 mol L−1HClO4aqueous solutions, which are defined in potential regions very close to each other. The adsorptionprocess of flavonoids on the modified electrode surface was carried out using an accumulation potentialof 0.55 V (vs. Ag/AgCl, 3 mol L−1KCl), and an accumulation time of 20 min.Considering that luteolin and rutin electrochemical responses show a high degree of overlapping, weprocessed the electrochemical signals using artificial neural networks. We used a supervised network,feed-forward network with Levenberg-Marquardt back propagation training. Values of 92.6 ± 0.4 and92 ± 1 mg per tablet were determined by the artificial neural networks methodology for luteolin andrutin, respectively. According to values declared by the manufacturer, differences of 7.4 and 8.0% werecalculated for luteolin and rutin, respectively. Results obtained with electroanalytical methodologieswere in very good agreement with those obtained by HPLC.