"Pyretroid-genetic resistance in the dengue vector (Aedes aegypti) in Posadas, Argentina"

FAY J.V.; ESPINDOLA S.; BOAGLIO V.; BLARIZA M.J.; LOPEZ K.; ZELAYA F.; KULKARNI M.; ARGÜELLES C.; FERRERAS J.; MIRETTI M.

Frontiers in Public Health, section Infectious Diseases: Epidemiology and Prevention.

Edited by: Thiago Vasconcelos Dos Santos

Lugar: Lausanne; Año: 2023

2296-2565

Pyrethroids are extensively used to control adult populations of the arboviral vector Aedes aegypti, rising concerns regarding theincreasing frequency and distribution of insecticide resistance mutations (kdr: knock down resistance) in the voltage gate sodiumchannel gene (Nav). Widespread use of pyrethroids impose a threat to the success of mosquito control and to the environment. Inthis work we investigated the presence of two kdr mutations (V1016I and F1534C) in the Nav gene and their distribution acrossfour neighbourhoods in Posadas, Argentina, with different Aedes abundance and contrasting socio-economic status (SES). Alleles ateach locus were interrogated using TaqMan SNP genotyping assays in DNA extracted from adult females collected in a longitudinalstudy. We report the presence of pyrethroid both resistance alleles (kdr 1016I=29.08%, kdr 1534C=70.70%) among adult females. Thecombined kdr genotypes frequency reveals that approximately 70% of local adult females have an enhanced resistance topyrethroids. The proportion of resistant adult females (with at least one kdr allele in each locus) and Aedes abundance both showedan uneven distribution between neighbourhoods with different SES (p