LPS from porphyromonas gingivalis affects trophoblast cell function and immune-trophoblast interaction.

MARIA DE MAR ROMÁN MUÑOZ; CALO GUILLERMINA; DAIANA VOTA,; FÁTIMA MERECH; DANIEL PAPARINI; RAMHORST ROSANNA; CLAUDIA PÉREZ LEIRÓS; VANESA HAUK

Congreso; Renión anual conjunta; 2018

Placentation is associated with trophoblast cell differentiation into invasive phenotypes,activation of matrix metalloproteinases (MMP) as well as trophoblast-immune cell interactionto maintain immune homeostasis. Porphyromonas gingivalis is considered to be an importantpathogen of periodontal disease that has been also implicated in adverse pregnancy outcome(APO). However the pathophysiology remains unknown.The aim of this study was to examined the effect of a virulence factor such aslipopolysaccharide from Porphyromonas gingivalis (PgLPS) on trophoblast cell function andtrophoblast-immune interaction. Cell migration was studied in the human trophoblastic cellline from 1st trimester Swan-71 by wound healing assays and invasiveness in Matrigel-coveredtranswells. MMPs, TIMPs and cytokine expression was measured by RTqPCR. Whole blood wasobtained from healthy donors and neutrophil purified on Ficoll-Triyosom gradient and Dextransedimentation. The effect of Swan-71 treated with PgLPS conditioned media (CM) onneutrophil migration through transwells was quantified by Flow cytometry.PgLPS treatment (10ng/ml) significantly reduced cell invasion with more than five timesincreased expression of tissue inhibitor of matrix metalloproteinase -1 and -2 (P