IMMUNOMETABOLIC REPROGRAMMING OF HUMAN NEUTROPHILS BY TROPHOBLAST FACTORS AND EARLY PREGNANCY

GUILLERMINA CALO; DAIANA VOTA; FLORENCIA SABBIONE; FÁTIMA MERECH; VANESA HAUK; LUCIANA DOGA; LUCIANA DERAMO; ALDO SQUASSI; ROSANNA RAMHORST; ANALIA TREVANI; CLAUDIA PÉREZ LEIRÓS

Congreso; Reunión Anual de la International Congress of Immunology (IUIS); 2023

BackgroundTrophoblast cells interact with maternal immune cells at placentation favoring an anti-inflammatorymicroenvironment required for fetal growth. We have previously shown that conditioned media fromtrophoblast cells (CM) induced a proangiogenic anti-inflammatory profile on neutrophils but theirimmunometabolic reprogramming in the context of pregnancy was not yet studied.Our aim was to evaluate the in vitro effect of trophoblast cell-derived factors on neutrophils’ metabolic,phenotypic, and functional profile and its correlate during early pregnancy.MethodsNeutrophils isolated from non-pregnant (NP-PMN) and 16–20 weeks pregnant women (P-PMN) werecultured with the classical stimulus PMA or with human first trimester trophoblast cell line HTR-8/SVneo conditioned media (CM). PMN functional profiles were assessed by qPCR and flow cytometry.Glucose uptake, lipid accumulation, fatty acids uptake and reactive oxygen species (ROS) productionwere determined using 2-NBDG, BODIPY 493/503, FL-C12 and DCFH-DA respectively.ResultsTrophoblast CM enhanced glucose uptake (P