INVESTIGADORES
APARICIO Jose Luis
artículos
Título:
CHANGES IN ANTIBODY SPECIFICITIES AND CYTOKINE RELEASE AFTER INFECTION WITH LACTATE DEHYDROGENASE-ELEVATING VIRUS
Autor/es:
JOSÉ L. APARICIO ; ANUBHA SAXENA; JEAN-PAUL COUTELIER; JACQUES VAN SNICK; LILIA A. RETEGUI
Revista:
INTERNATIONAL IMMUNOPHARMACOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 15 p. 544 - 549
ISSN:
1567-5769
Resumen:
Lactate dehydrogenase-elevating virus (LDV) is an apparently innocuous and persistent virus that can modify
mouse immune reactions. We have shown that LDV-infected mice immunized with human growth hormone
(hGH) showed a deep modification of the specificity of the anti-hGH antibodies (Ab) in CBA/Ht mice but not
BALB/c animals. The aim of this work was to extend the previous observations to another mouse strain,
C57BL/6, as well as to an antigen unrelated to hGH, ovalbumin (OVA), and to explore at the same time the
production of various cytokines at serum and cellular levels. The amount of Ab directed to hGH or OVA native
antigenic determinants versus the concentration of Ab to cryptic epitopes was evaluated by ELISA competition
experiments. Results indicated that LDV infection affected Ab specificity solely in CBA/Ht mice.
In CBA/Ht the virus infection was associated with a reduction of the Ab titers to hGH native epitopes and with
a decrease of IL-13 and IL-17 serum levels, but Ab to native OVA epitopes were increased with a simultaneous
increase of IL-17. Accordingly, only lymph node cells from infected CBA/Ht mice immunized with OVA were
found to produce INF-γ, IL-13 and IL-17.
Thus, a correlation of cytokine production with a change in Ab specificity after a viral infection was found, although
this phenomenonwas restricted to a given antigen and to the genetic background of immunized animals.
These observations suggest that an apparent harmless virus can affect some immunological mechanisms, which
could lead, for example, to inflammatory or autoimmune disorders.