Genetic diversity of Fusarium graminearum isolates obtained from wheat associated to Fusarium Head Blight from diverse geographical locations of Argentina

VERONICA FABIANA CONSOLO; LEONEL ORTEGA; GRACIELA SALERNO; ANDREA ASTORECA; TERESA ALCONADA

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2015

0325-7541

Fusarium Head Blight is an important wheat disease in Argentina Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was studied by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands being 30 polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers has shown genetic variability among the analyzed isolates, however IGS-RFLP was more efficient than ISSR with higher polymorphism average (59.91%) than the second (44.11%). The averages of polymorphism information content (PIC) value were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Clustering of genotypes within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability using IGS-RFLP and ISSR markers of F. graminearum isolates from wheat in Argentina.