INVESTIGADORES
RICARDI Martiniano Maria
congresos y reuniones científicas
Título:
Screening of essential genes for cold growth in Pseudomonas extremaustralis
Autor/es:
TRIBELLI, PAULA M; SOLAR VENERO ESMERALDA C; MARÍA GÓMEZ-LOZANO; MARTINIANO RICARDI; SOREN MOLIN; NANCY I.LOPEZ
Lugar:
Ciudad Autonoma de Buenos Aires
Reunión:
Workshop; Global Exchange lectures. EMBO course: Bacterial small RNAs.; 2014
Institución organizadora:
EMBO
Resumen:
Species belonging to the Pseudomonas genus represent an important proportion of the bacteria that could be found in different environments. Pseudomonas extremaustralis is a non-pathogenic species isolated from an Antarctic environment and its genome has been recently obtained. This species shows high stress resistance like cold, freezing, oxidative stress and hydrocarbon tolerance and degradation. Also presents several genomic islands and other mobile elements suggesting an important genomic plasticity. The growth under low temperatures requires several adaptations in different cellular processes to cope with the stressful factors present under cold conditions. These adaptations include changes in membrane biosynthesis, oxidative stress resistance and biofilm formation for example. Although some works have studied some genes in the adaptability to cold conditions, there are few works including global genetic studies with native bacteria from an extreme environment. We are focused in the determination of essential genes for growth under cold conditions and in the molecular mechanism involved. As experimental strategy we are performing a random mutant library and transcriptional profiles. We have identified at least 5 essential genes involved in survival under cold conditions and the transcriptional profile at 28○C and 10○C showed an important number of genes differentially expressed. But our challenge now is understand the role of non-coding RNA under low temperatures. Due the ecological and biotechnological importance of bacteria not only in the environment but also in different human activities, the understanding of the cellular and molecular mechanisms related to the adaptability to extreme conditions is crucial for basic and applied knowledge.