INVESTIGADORES
RICARDI Martiniano Maria
congresos y reuniones científicas
Título:
Screening of essential genes for cold growth in Pseudomonas extremaustralis
Autor/es:
TRIBELLI, PAULA M; SOLAR VENERO ESMERALDA C; MARÍA GÓMEZ-LOZANO; MARTINIANO RICARDI; SOREN MOLIN; NANCY I.LOPEZ
Lugar:
Ciudad Autonoma de Buenos Aires
Reunión:
Workshop; Global Exchange lectures. EMBO course: Bacterial small RNAs.; 2014
Institución organizadora:
EMBO
Resumen:
Species
belonging to the Pseudomonas genus represent an important proportion of the
bacteria that could be found in different environments. Pseudomonas
extremaustralis is a non-pathogenic species
isolated from an Antarctic environment and its genome has been recently
obtained. This species shows high stress resistance like cold, freezing,
oxidative stress and hydrocarbon tolerance and degradation. Also presents
several genomic islands and other mobile elements suggesting an important
genomic plasticity.
The
growth under low temperatures requires several adaptations in different
cellular processes to cope with the stressful factors present under cold
conditions. These adaptations include changes in membrane biosynthesis,
oxidative stress resistance and biofilm formation for example. Although some
works have studied some genes in the adaptability to cold conditions, there are
few works including global genetic studies with native bacteria from an extreme
environment.
We
are focused in the determination of essential genes for growth under cold
conditions and in the molecular mechanism involved. As experimental strategy we
are performing a random mutant library and transcriptional profiles. We have
identified at least 5 essential genes involved in survival under cold
conditions and the transcriptional profile at 28○C and 10○C showed an important
number of genes differentially expressed. But our challenge now is understand
the role of non-coding RNA under low temperatures. Due the ecological and
biotechnological importance of bacteria not only in the environment but also in
different human activities, the understanding of the cellular and molecular
mechanisms related to the adaptability to extreme conditions is crucial for
basic and applied knowledge.