Pretreatment Combination Reduces Remote Organ Damage Secondary to Intestinal Reperfusion Injury in Mice: Follow-up Study

STRINGA, P; LAUSADA, N; ROMANIN, D; PORTIANSKY, E; ZANUZZI, C; MACHUCA, M; GONDOLESI, G; RUMBO, M

TRANSPLANTATION PROCEEDINGS

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2016 vol. 48 p. 210 - 216

0041-1345

Background. Intestinal ischemia-reperfusion injury occurs after different surgicaltreatments, including intestinal transplantation. This harmful process may have an effectin remote organs, leading to multiple organ dysfunction syndrome and death. Therefore,to establish strategies to attenuate local and remote damage constitutes a challenge forexperimental and clinical surgeons in the intestinal surgical field.Methods. We evaluated the effect of ischemic preconditioning and tacrolimus pretreatmentapplied alone and in combination against local and remote damage caused by prolongedintestinal ischemia-reperfusion injury in a mouse model of warm ischemia.Results. Ischemic preconditioning applied alone and in combination with tacrolimusdecreased histological damage (P < .05), number of apoptotic cells (P < .05), nitrosativestress (P < .01), and serum lactate dehydrogenase activity (P < .05) and lowered uremia(P < .05) compared with untreated post-reperfused intestines. Regarding remote organdamage, combination therapy was the unique condition able to attenuate lung (mainlyneutrophil infiltration and hemorrhage), liver (sinusoidal congestion and hepaticvacuolization), and kidney (acute tubular necrosis and hydropic degeneration)histological alterations (P < .05), compared with the untreated group.Conclusions. These results support the application of these strategies in combination tominimize the impact of ischemia-reperfusion injury in the whole organism as a strategy toprevent multiple organ dysfunction syndromes and minimize the clinical impact.