Advanced maternal age increases lipid oxidative damage of the decidua during early pregnancy in rats

LAURA LEONARDI, CINTIA GATTI, EVANGELINA CAPOBIANCO, ALICIA JAWERBAUM ALICIA, ROMINA HIGA.

Workshop; REDOX BA 2023 Workshop on Redox Nutrition and Toxicology. Buenos Aires, Argentina.; 2023

Background and Aims: Pregnancy at Advanced Maternal Age (~35 years, AMA) induces obstetric complications and neonatal adverse outcomes. During early pregnancy, an optimal decidual function is essential for a correct placenta and embryo development. FoxO1 is a transcription factor that plays a role in decidualization, placentation and embryo development by modulating genes related to oxidative stress and cell cycle withdrawal. FoxO1 can be inhibited by phosphorylation. Aim: To evaluate reproductive alterations in a rat model of AMA during organogenesis stage. In decidua of AMA rats analyze lipoperoxidation and mRNA levels of proteins of electron transport chain (ETC), FoxO1 activation and the expression of its target gene p21. Methods: 3 months old (Control) and 10 months old (AMA) Wistar rats were mated with young males. Decidua were obtained on day 12 of pregnancy to measure FoxO1 phosphorylation status (Western Blot), mRNA levels of p21 (senescence marker), Ndufa6 and Nd1 (ETC) (RT-qPCR), lipoperoxidation (TBARS) and 4-HNE (immunohistochemistry).Results: AMA rats showed an increase in macroscopic uterine anomalies (+41%, p