The miR-122-SIRT1-PPARalpha pathway in the decidualized uteri from prepuberal offspring of diabetic rats: effect of a maternal diet enriched in olive oil

CINTIA ROMINA GATTI, FLORENCIA SCHIBERT, ROMINA HIGA, ALICIA JAWERBAUM.

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2023

Introduction: Reduced markers of decidualization and an increased prooxidant environment have been previously found in the decidualized uteri of the offspring of diabetic rats, an alteration prevented by a maternal diet enriched in olive oil. In other systems, a pathway that involves the microRNA-122 (miR-122), the sirtuin SIRT1 and the nuclear receptor PPARα regulates the prooxidant/proinflammatory environment. Aim: To evaluate the miR-122-SIRT1-PPARα pathway in the decidualized uteri of prepuberal offspring of diabetic rats fed or not with an olive oil-enriched diet. Methods: A mild pregestational diabetic rat model was induced in F0 females by neonatal administration of streptozotocin (90 mg/kg sc). Control and diabetic females were mated with healthy males and received a 6% olive oil-enriched diet or a standard diet from day 1 of pregnancy until parturition. The offspring were fed a standard diet and the uteri of the female offspring (F1) were evaluated on postnatal day 30, after induction of decidualization with PMSG (50 UI) and hCG (50 UI). MiR-122, SIRT1 and PPARα was evaluated by RT-qPCR. Results: The offspring of diabetic rats (DG) showed a reduction in miR-122 levels in the decidualized uteri (-89%, p