Effect of dye localization and self-interactions on the photosensitized generation of singlet oxygen by rose bengal bound to bovine serum albumin

ESPECHE TURBAY, MARÍA BEATRIZ, VALENTINA REY, NATALIA M. ARGAÑARAZ, FAUSTINO E. MORÁN VIEYRA, CLAUDIO D. BORSARELLI; VALENTINA REY; NATALIA M. ARGAÑARAZ; FAUSTINO E. MORÁN VIEYRA; ALEXIS ASPEÉ; EDUARDO A.LISSI; LUIS A. DORADO, VALENTINA REY, ANA LEDESMA, BEATRIZ LOPEZ DE MISHIMA, CARLA E. GIACOMELLI, CLAUDIO D. BORSARELLI

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2014

1011-1344

The spectroscopic and photophysical properties of rose bengal (RB) encased in bovine serum albumin (BSA) have been examined to evaluate the photosensitized generation of singlet molecular oxygen (1O2). The results show that RB photophysical and photosensitizing properties are highly modulated by the average number of dye molecules per protein (n). At n 1, nonspecific binding of up to »6 RB molecules per BSA is produced, allowing efficient static quenching of excited states of RB preventing photosensitization of 1O2. These results provide useful information for development of dye-protein adducts suitable for using as potential intracellular photosensitizers.