A circular microchannel with integrated electrodes for DNA electrophoresis

LERNER, BETIANA; KLER, PABLO A.; ORDOÑEZ ARIAS, A. F.; PEREZ, MAXIMILIANO; LASORSA, CARLOS; BERLI, CLAUDIO L. A.

MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS

SPRINGER

Lugar: Berlin; Año: 2012 vol. 19 p. 739 - 742

0946-7076

The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedded electrodes provide several advantages in relation to conventional linear microchannels. The paper describe the design, modeling, construction, and testing of the micro device. Numerical simulations were used to investigate the electric field into the microchannel. In addition, the electrophoretic transport of DNA samples was studied under different voltage configurations. The experiments reported show the functionality of the device, and illustrate interesting features of DNA transport.