The role of sphingosine kinases on chronic lymphocytic leukemia

MARICEF VERGARA RUBIO; ELÍAS ESTEBAN ENRIQUE; GREGORIO CORDINI; ANA COLADO; DENISE RESNIK; MORANDE PABLO; BEZARES FERNANDO; BORGE MERCEDES; GIORDANO MIRTA; GAMBERALE ROMINA

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

SAI

Sphingosine kinases (SK1 and SK2) produce sphingosine-1-phosphate which mediates cell growth,survival and differentiation, among other functions. We previously reported that inhibition of SK1by the commercial inhibitor SKI-II, reduces leukemic cell survival, activation and proliferation inCLL patients (Almejún, Haematologica-2017). The aims of this study were: 1) to evaluate the effectof SKI-II on non-leukemic lymphocytes, and 2) to determine whether SK2 regulates leukemic cellsurvival. Then, peripheral blood mononuclear cells (PBMC) from CLL patients were cultured withdifferent doses of SKI-II, and NK and T cell viability was evaluated by flow cytometric alterationsof light-scattering properties at 24hs. We found that SKI-II reduces T and NK cell survival in a dosedependent way (n=15, p