Análisis de la expresión de la enzima autocatalitica UDP-glucosa proteína transglucosidasa.

VERÓNICA DE PINO; FLAVIA WALD; RALPH KISSEN; PATRICK DU JARDIN; SILVIA MORENO

Punta del Este. Uruguay

Congreso; XI Reunión Latinoamericana de Fisiología Vegetal.; 2002

Sociedad Argentina de Fisiologia Vegetal. Facultad de Agronomia-Facultad de Ciencias.Universidad de la Republica Oriental del Uruguay

Expression Analysis of the autocatalytic glycosyltransferase UDP-Glucose: Protein Transglucosylase Verónica De Pino, Flavia Wald, Ralph Kissen, Patrick Du Jardin and Silvia Moreno   Plant cells, unlike animal cells, are surrounded by a cell wall and studies aimed at identifying the protein responsible for biosynthesis of the cell wall polysaccharides have revealed the presence of a small multigene family of autocatalytic glycosyltransferases. Uridine diphosphate glycosyltransferases (UGTs) mediated the transfer of glycosyl residues for activated nucleotide sugars to acceptor molecules, thus regulating properties of the acceptors such as their solubility and transport within the cell. UDP-Glucose: Protein Transglucosylase is a member of a little subgroup of UGTs that catalyses the transfer of glucose, xylose and galactose from the respective UDP-sugar nucleotides to itself. Although is it thought to have a role in hemicellulose synthesis, it function is unknown. We have been interested in elucidating the regulation of UPGT in order to identified putative genes involved in the regulation polysaccharide synthesis. Previously, we studied the functional expression of one potato UPTG isoform in E.coli and here, we studied the functional expression of a second cDNA of the enzyme. Northern blots analyses showed a correlation between UPTG transcript leves and the growing states of in vitro potato and rice plant tissues, in which there was an active synthesis of cell wall components. Also, we observed that UPTG is modulated by several hormonal factors in rice in vitro plantlets