Potencial adhesion ability to ephitelial cells by Saccharomyces cerevisiae mycotoxin adsorbents strains

ARMANDO M.R.; PIZZOLITTO R. P.; PEIRANO M.S.; SALVANO M.; ESCOBAR F.; SABINI L.; DALCERO A.M.; CAVAGLERI L.R.

Tafí del Valle (Tucumán)

Jornada; XXV Jornadas científicas, Asociación de Biología de Tucumán; 2008

Soc. Biología de Tucumán

Mycotoxins are toxic metabolites produced by fungi in feed. The presence at levels above the tolerable represents a threat to food safety and a significant risk in human and animal health. Some strategies have been developed to reduce the mycotoxins contamination. One promising alternative is the use of functional foods containing probiotics capable of exerting beneficial effects in animals. Saccharomyces cerevisiae has been supplied as food by their huge profits and constitutes a potential tool for the removal of mycotoxins in animal feed. Adherence to epithelial cells is a prerequisite for temporarily persistence in the host and a key mechanism to carry out his probiotic activity. The aim of this study was to determine the potential for S. cerevisiae strains to adhere to Vero cells. The strains of S. cerevisiae used in this study were isolated from the pig ecosystem and evaluated in their ability to aflatoxin B1 and ochratoxin A detoxification. The test was conducted following the methodology proposed by Kumura et al. (2004). The results indicate that the adherence ability of S. cerevisiae vary among strains. We have observed adherence to Vero cells of 100% of studied strains. The  RC009, RC012 and RC017 are the most adherent strains.