INVESTIGADORES
ANCHORDOQUY Juan Mateo
artículos
Título:
Reproductive hormones influence zinc homeostasis in the bovine cumulus-oocyte complex: Impact on intracellular zinc concentration and transporters gene expression
Autor/es:
PASCUA, ANA M.; NIKOLOFF, NOELIA; CARRANZA, ANA C.; ANCHORDOQUY, JUAN P.; QUINTANA, SILVINA; BARBISÁN, GISELA; DÍAZ, SILVINA; ANCHORDOQUY, JUAN M.; FURNUS CECILIA
Revista:
THERIOGENOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2020 vol. 146 p. 48 - 57
ISSN:
0093-691X
Resumen:
Zinc (Zn) is a vital trace element for the body and its bioavailability influences numerous reproductiveevents. However, the mechanisms that regulate Zn homeostasis in the cumulus-oocyte complex (COC) are yet to be elucidated. The aim of this study was to investigate the role of estradiol 17-beta (E2), FSH and LH in Zn homeostasis regulation in bovine COC matured in vitro and Zn transporters gene expression.For this purpose, intracellular Zn levels in oocytes and cumulus cells (CC) were assessed using a Znspecific fluorescent indicator. In addition, gene expression and sequencing of six Zn transporters (Slc39a6, Slc39a8, Slc39a14, Slc30a3, Slc30a7 and Slc30a9) were assessed. Our results demonstrated that the simultaneous presence of E2, FSH, and LH during oocyte maturation altered intracellular zinc levels and transporters expression in both oocytes and CC. Transporter?s gene expression was different in oocytes and CC, possibly due to cell-specific changes in Zn levels during maturation. The interactioneffects of Zn with hormonal treatments influenced the results. This study emphasizes that Slc39a6 is highly sensitive to hormone induction. Overall, the hormonal modulation of Zn homeostasis in the COC was evidenced. Also, a preponderant role of FSH as a modulator of Zn intracellular levels and transporter gene expression is suggested.