THE SPLEEN TYROSINE KINASE SYK PARTICIPATES IN THE ACTIVATION OF GAMA DELTA T CELLS BY MONOSODIUM URATE CRYSTALS

CAROLINA MAIUMI SHIROMIZU; TOWSTYKA NADIA YASMÍN; COLADO ANA; IRENE KEITELMAN; FLORENCIA SABBIONE; BORGE MERCEDES; TREVANI ANALÍA; JANCIC CAROLINA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

gd T cells act as sensors of cellular stress and infection. They recognizedanger and pathogen-associated molecular patters, such asphosphoantigens. Uric acid is a cellular stress signal, which is releasedfrom dying cells and promotes the generation of innate andadaptive immune responses. The inflammatory effect of uric aciddepends on its precipitation into monosodium urate (MSU) crystals.MSU crystals activate different intracellular pathways, i.e. p38MAPK, Syk kinase and PI3K. We have previously reported that MSUcrystals activate gd T cells and that this activation was reduced inpresence of a Syk tyrosine kinase inhibitor. Now, we aim to investigatemore in detail the participation of Syk tyrosine kinase during theintracellular signaling triggered by MSU crystals in gd T cells. To thispurpose, gd T cells were purified from human peripheral blood mononuclearcells by using an anti-TCR gd MicroBead isolation kit. Afterpurification, gd T cells were incubated or not with MSU crystals (200μg/ml) at different time points, and then we analyzed the expressionof phospho(p)-Syk (the active form of the tyrosine kinase) by immunofluorescenceand confocal microscopy analysis. We observed anincrease in p-Syk expression at 5 and 15 min post-stimulation byMSU crystals (p