Chronic Lymphocytic Leukemia increase neutrophil survival and promote their differentiation into CD16high CD62Ldim sub-set

PODAZA ENRIQUE; RISNIK DENISE; COLADO ANA; ELÍAS, ESTEBAN E.; ALMEJÚN MARÍA BELÉN; FERNANDEZ GRECCO HORACIO; BEZARES FERNANDO; BORGE MERCEDES; GAMBERALE ROMINA; GIORDANO MIRTA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Chronic lymphocytic leukemia (CLL) is characterizedby the progressiveaccumulation of clonal B lymphocytes in blood,lymph nodes, spleen and bone marrow. Within lymphoid organs,CLL cells foster a protective microenvironment where they surviveand proliferate. While almost every cell type in lymphoid tissueswas reported to be modified by the leukemic clone, there is scarceinformation regarding the interaction of CLL cells with neutrophils.Tumor associated neutrophils (TANs), described in multiple typesof solidtumors, facilitate cancer progression. Our aim was to determineif CLL cells were capable ofmodifying neutrophil phenotypeand survival. To that purpose, neutrophils from healthy donor(HD) were co-cultured with purified leukemic cells from CLL patients(ratio 1:1) and apoptosis was assessed by flow cytometry usingAnnexin-V. Our results showed that CLL cells delayed neutrophilapoptosis at 24-72 h (n=15, p