REFINEMENT OF THE TECHNIQUE OF DRUG ADMINISTRATION THROUGH THE APPLICATION OF ELECTROSPUN TRANSDERMAL MEMBRANES

ECHARTE, STELLA MARIS; ORALLO, DALILA; CÁMARA, CAROLINA ANAHÍ; LA COLLA, ANABELA; RIVERO, GUADALUPE; CHISARI, ANDREA NANCY

Mar del Plata

Congreso; Reunión Conjunta SAIC SAI FAIC SAFIS 2022; 2022

SAIC-SAI-SAFIS

The controlled release of bioactive agents can be achieved fromtheir encapsulation in a porous matrix in a controlled or sustainedmanner over time, generally by diffusion. The use of electrospuncellulose acetate membranes as transdermal patches with vitaminsE has been reported, and their in-vitro release has been characterized.However, no in vivo studies have been carried out. Polymericcontrolled release systems allow evaluating different dosesand modulating their release over time, through the alteration of thegeometry, architecture, size, surface properties and composition ofthe membranes. We aimed to prepare and characterization of nanofibrousmatrices electrospun cells loaded with vitamin E for invivouse as transdermal patches in wound healing in a Wistar rat model.Polymeric solutions of poly(ε-caprolactone) (PCL, 80000Da) at 15-20% w/v were prepared. Different concentrations of Vitamin E wereincorporated 1% (A); 6% (B) and 12% (C). The punch techniquewas used to generate the wound in the middle dorsal part of the rat.A membrane disk was placed directly over the area to be healed.The efficient incorporation of vitamin E into the membranes wasobtained by Agilent 8453 UV-visible absorption spectroscopy, performinga calibration curve with Vitamin E as standard compound at296 nm. Morphological characterization was performed by scanningelectron microscopy (SEM). Regarding the in vivo model, the diameterof healing over time was measured and histological sections ofskin were made and stained with the H&E technique. The followingresults of mg Vit E/g membrane were obtained for (A) 4.90±0.02; (B)16.86±0.05 and (C) 46.05±0.03. We observed that the diameter ofthe wound decreases faster in the membranes containing (B) and(C). Regarding histology greater amount of scar tissue is associatedhigher concentration of vitamin. In conclusion, improvements in thehealing process are observed, which demonstrates the effectivenessof the membranes in drug dosing.