INVESTIGADORES
DE ROSSI Maria Cecilia
artículos
Título:
Dynamics of Intracellular Processes in Live-Cell Systems Unveiled by Fluorescence Correlation Microscopy
Autor/es:
NICOLAS GONZALEZ BARDECI; JUAN FRANCISCO ANGIOLINI; MARÍA CECILIA DE ROSSI; LUCIANA BRUNO; VALERIA LEVI
Revista:
International Union of Biochemistry and Molecular Biology
Editorial:
Wiley Online Library
Referencias:
Lugar: Estados Unnidos; Año: 2016 vol. 69 p. 8 - 15
ISSN:
1521-6551
Resumen:
Fluorescencefluctuation-based methods are non-invasive microscopy tools especially suitedfor the study of dynamical aspects of biological processes. These methodsexamine spontaneous intensity fluctuations produced by fluorescent moleculesmoving through the small, femtoliter-sized observation volume defined inconfocal and multiphoton microscopes. The quantitative analysis of theintensity trace provides information on the processes producing thefluctuations that include diffusion, binding interactions, chemical reactionsand photophysical phenomena. In this review, we present the basic principles ofthe most widespread fluctuation-based methods, discuss their implementation instandard confocal microscopes and briefly revise some examples of their applicationsto address relevant questions in living cells. The ultimate goal of thesemethods in the Cell Biology field is to observe biomolecules as they move,interact with targets and perform their biological action in the naturalcontext.
