Evaluation of DNA damage and oxidative DNA damage in mothers of newborns babies with congenital anomalies from Santa fe, Argentina.

COLUSSI, CARLINA; BASCELLI BC; SOFICCI, V.; FUNES S.; POLETTA, GISELA L.; SIMONIELLO, MA. FERNANDA

Asunción del Paraguay

Congreso; XI Congreso Latinoamericano de Mutagénesis, Carcinogénesis y Teratogénesis Ambiental. I Jornada de la Sociedad Paraguaya De Genétic; 2019

ALAMCTA

Maternal exposure during pregnancy to chemicals, alcohol, tobacco, and some infections, may increase the risk of having a newborn affected by congenital anomalies (CA). The aim of this study was to evaluate genotoxicity in mothers of newborn with and without CA (cases and controls, respectively). A face to face questionnaire allowed to obtain information on demographic data and individual lifestyle were performed. Comet assay was applied in peripheral blood samples to determinate DNA damage (Comet) and oxidative DNA damage with Endonuclease III enzyme (Endo and Endo sites: Endo subtraction Comet) in 173 woman (25.2 ± 5.8 years old). No significant DNA damage was observed between case and control groups for three variables: Comet (p=0.7), Endo (p=0.3) and ENDO sites (p=0.3). In Control group, fruit and vegetables intake and physical activity during pregnancy indicated less DNA damage and oxidative DNA damage (Comet p=0.05; Endo p=0.03 and Comet p=0.04; ENDO sites p=0.009, respectively). In Controls, active or passive smoking, and alcohol drinking habit during pregnancy showed slightly higher DNA damage, but not statistically significant (p>0.05). In Case group, no significant differences were observed considering these habits. The results obtained for mothers of newborns with CA were useful to improve the characterization of individual risk and encourage us to continue working to understand the mechanisms involved in this particular group.