Expression pattern of antioxidant enzymes genes in Caiman latirostris hatchlings exposed to pesticide mixtures.

ODETTI, LUCÍA; PARAVANI, ENRIQUE; SIMONIELLO, MA. FERNANDA; POLETTA, GISELA L.

Asunción del Paraguay

Congreso; XI Congreso Latinoamericano de Mutagénesis, Carcinogénesis y Teratogénesis Ambiental. I Jornada de la Sociedad Paraguaya De Genétic; 2019

ALAMCTA

Pesticide exposure of Caiman latirostris natural populations is of great concern, considering the effects we reported previously on genotoxicity and oxidative stress. Recently, a new family of biomarkers was developed for the analysis of gene expression related to cellular stress, which can be used as early warning sensors of xenobiotics exposure. The aim of this study was to validate cat and sod genes involved in the antioxidant response and quantify their expression levels in peripheral blood of C. latirostris after embryonic exposure to pesticide mixtures of Cypermethrin, Chlorpyrifos and Glyphosate based formulations. Treatments were: vehicle control treated with ethanol; one group exposed to the mixture of the three formulations (Mx3: CYP -0.12 % + CPF-0.8% + RU-2%) and three groups exposed to the binary mixtures of them (M1: RU + CYP; M2: CPF + CYP; M3: CPF + RU). The concentrations applied for each compound corresponded to that recommend for soy crops and applied on the nest material in contact with the eggs. After hatching, total RNA was isolated from blood through a protocol adapted for C. latirostris by our research group. Results showed a statistically significant increase in expression level of cat for M3 and of sod for M1 and M3, compared to control (p