Embryotoxicity evaluation of the insecticide Chlorpyrifos and pesticide mixtures on Caiman latirostris (Broad-snouted caiman), through biomarkers of genotoxicity

LÓPEZ GONZÁLEZ, EVELYN; ROMITO, MA. LAURA; SIROSKI, PABLO A.; POLETTA, GISELA L.

Buenos Aires

Congreso; SETAC Latin America 11th Biennial Meeting; 2015

Society of Environmental Toxicology and Chemmistry Capítulo Argentina

The aim of this study was to evaluate the effects of embryonic exposure to different concentrations of the widely used commercial formulation Chlorpyrifos (Lorsban 48E® -CPF, 48%) and a complex pesticide mixture consisiting of Cypermethrin (Atanor® -CYP, 25%) + Glyphosate (Roundup Full II® -GLY, 66.2%) + Chlorpyrifos (Lorsban* 48E® -CPF, 48%) on C. latirostris.The study was carried out under the ?Proyecto Yacaré? (Gob. Santa Fe/MUPCN) facilities. Experiments were performed on embryos of C. latirostris exposed by topication. Eggs were randomly distributed into seven experimental groups (N= 84; 12 eggs per experimental group with two replicates of 6 eggs each): 1) a negative control (NC) treated with distilled water; 2) a vehicle control (VC) treated with ethanol; 3-6) 4 groups exposed to 1, 10, 100, and 1000 µg/ egg of CPF formulation; 7) a complex mixture (M) treated with: 10 µg/egg of CPF (Lorsban 48E®, 48%) + 10 µg/egg of CYP (Atanor®, 25%) + 500 µg/egg of GLY(Roundup Full II®, 66.2%). The eggs were incubated in an artificial incubator, under controlled conditions of temperature of 31 ± 1 °C and 95% humidity.At the end of incubation period, hatching success was registered and blood samples were obtained from the spinal vein of all neonates for the application of genotoxicity biomarkers on erythrocytes: Micronucleus test and Comet assay.The results showed genotoxic damage at all concentrations of CPF tested and the mixture, respect to NC (p