Micronuclei and nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) after exposure to different pesticide formulations during development

LÓPEZ GONZÁLEZ, EVELYN; LARRIERA, ALEJANDRO; SIROSKI, PABLO A.; POLETTA, GISELA L.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2017 vol. 136 p. 84 - 91

0147-6513

This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) and Nuclear abnormalities (NAs) tests in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application through the eggshell.Embryos were exposed to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000 µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000 µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN and NAs tests in erythrocytes, as markers of genotoxicity. NAs included buds, binuclei (BiN), notched nuclei (NN), eccentric nuclei (EN) and total nuclear abnormalities (TNA).Results indicated a significant increase in the frequency of MN for, PANZ1000, END 10, CYP 1, and CYP 100 (p