Total and differential white blood cells count in Caiman latirostris after in ovo and in vivo exposure to insecticides. J. Immunotoxicol. IF: 1,989; SJR: 0,662= Q3 (Toxic

LATORRE, MA. AGUSTINA; ROMITO, MA. LAURA; LARRIERA, ALEJANDRO; POLETTA, GISELA L.

JOURNAL OF IMMUNOTOXICOLOGY

INFORMA HEALTHCARE

Lugar: London; Año: 2016

1547-691X

Agricultural activities associated mainly with soybean crops affect the natural environment and wildlife by habitat destruction and the extensive use of agrochemicals. The aim of this study was to evaluate immunotoxic effects of the insecticides cypermethrin and endosulfan in Caiman latirostris analyzing total (TWBC) and differential white blood cell count (DWBC) after in ovo and in vivo exposure. Eggs (in ovo) and hatchlings (in vivo) from nests harvested in natural habitats were artificially incubated and reared under controlled conditions in the Proyecto Yacaré (Gob.Santa Fe/MUPCN) facilities. Exposure of embryos was performed by topication on the eggshell during the first stage of development. The treatments were: distilled water (negative control; NC), ethanol (vehicle control; VC), four groups treated with different concentrations of cypermethrin (CYP) and four groups with endosulfan (END). In vivo exposure was performed by immersion; treatments were: NC, VC, two groups exposed to CYP and two to END. After embryonic exposure to the insecticides, no differences were found in TWBC or DWBC among the neonates exposed to pesticides vs. controls. In the in vivo scenario, similar results were obtained for TWBC, but DWBC data showed differences between NC hatchlings and CYP-1 hosts for for heterophil, lymphocyte, and monocyte levels, and between NC and END-1 hosts for lymphocyte and monocyte levels. These results indicated that use of these insecticides may alter some aspects of the immune system of neonatal and yearling C. latirostris, causing instability of defense barriers and increasing their potential susceptibility to certain infections.