Relative expression of genes associated with adhesion to bovine mammary epithelial cells by Streptococcus uberis

FESSIA, ALUMINÉ S.; DIESER, SILVANA A.; RENNA, MARÍA SOL; RASPANTI, CLAUDIA G.; ODIERNO, LILIANA M.

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2020 vol. 132 p. 33 - 41

0034-5288

Streptococcus uberis is one of the most prevalent environmental pathogens of bovine mastitis. The S. uberis ability to adhere to mammary gland epithelia might provide an advantage to colonize the lactating mammary gland. Theobjectives of this study were (a) to select S.uberis strains according to their ability to form biofilm, (b) to determine adherence to and internalization into MAC-T cell and (c) to investigate the expression profile adherence genes in these S. uberis strains. For the assays, the MAC-T bovine mammary epithelial cell line was used. Relative expression of genes acdA, lmb, scpA, sua, fbp and lbp was quantified by RT-qPCR. We observed that the RC38 strain from clinical mastitis bovine showed in the six genes higher values than control in both conditions. While the strain with greater ability to adhere, from clinical mastitis and biofilm producer (RC29) evidenced higher values in group 1 (G1) (bacteria after the initial contact with MAC-T cells) and decrease in group 2 (G2) (adherent and internalized bacteria) than control. Strains with a moderate or strong capacity for biofilm production showed significantly lower relative expression values in the G2. In all adherence associated genes, strain RC19 showed relative expression values incremented in G1, while in G2 decreased expression. In conclusion, we did not find a single profile of relative expression because the relative expression levels of each gene differed depending on the strain and the co-culture stage of S. uberis cells fromwhich RNA was obtained