Macroencapsulation of Limosilactobacillus reuteri DSPV002C as nutritional supplement for piglets: Storage stability and survival in gastrointestinal conditions

ZIMMERMANN, JORGE ALBERTO; SIRINI, NOELÍ; OLIVERO, CAROLINA RAQUEL; RENNA, MARÍA SOL; SIGNORINI, MARCELO LISANDRO; ZBRUN, MARÍA VIRGINIA; FRIZZO, LAUREANO SEBASTIÁN; SOTO, LORENA PAOLA

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Año: 2023

0325-7541

The aim of this study was to evaluate the protective effect of the encapsulationof Limosilactobacillus reuteri DSPV002C in macrocapsules made from industrial materials dur-ing production, storage and under simulated gastrointestinal conditions in vitro and in vivo.The production of macrocapsules involved the evaluation of different wall materials (matrix),namely, gelatin and pregelatinized starch, different inoculums, matrix ratios, and diverse cry-oprotectants (whey permeate and maltodextrin). The different macrocapsules were arrangedin molds of similar size to pig pelleted food and lyophilized. Then, the viability of the macrocap-sules was assessed over time during storage at different temperatures (freezing, refrigerationand room temperature) and atmospheres (vacuum and non-vaccum). The macrocapsules with10% w/v gelatin + 5% w/v pregelatinized starch, permeated (10%, w/v), with a 9:1 inocu-lum:matrix ratio (GS7.5P9), stored under freezing conditions and vacuum, exhibited the highestviability of L. reuteri DSPV002C (9.3 log CFU/cap until 210 d). Under simulated gastrointesti-nal conditions, the encapsulated inoculum showed less viability loss (0.58 ± 0.09 log CFU/ml,26.53%), compared to the free culture (1.56 ± 0.16 log CFU/ml, 2.85%). Finally, by adminis-tering GS7.5P9 to pigs, the tolerance of the bacteria to the gastrointestinal environment wasverified, with viable counts equal to or greater than 3.72 log CFU/g of fecal matter throughout the trial. In this study, a high-density carrier probiotic macrocapsule of L. reuteri DSPV002Cwas obtained, which displayed a long shelf life, a suitable shape to be included in pig feed andan adequate survival of viable cells at the site of action.