Production of Laccases from Agricultural Wastes: Strain Isolation and Selection, Enzymatic Profiling, and Lab-Scale Production

AHMED, P.M.; PAJOT, H.F.; FERNÁNDEZ, P.M.

Mycoremediation Protocols

Springer

Lugar: New York; Año: 2022; p. 139 - 159

Fungal laccases (copper-containing oxidases) have profound applications in bioremediation and various other industrial and biotechnological areas. This chapter outlines recent developments in laccase production technologies, with a focus on the selection of the most promising producing fungal species, general considerations on media components and culture conditions, lab-scale production, and partial purification of enzymes. Furthermore, protocols related to solid-state fermentation (utilizing agriculture wastes), such as one-step affinity chromatography (employing a mesoporous material as support), and electrophoresis (used to check the enzyme purity), are described.