Targeted Anti-Inflammatory Peptide Delivery in Injured Endothelial Cells Using Dermatan Sulfate/Chitosan Nanomaterials

BLACHMAN A.; FUNEZ F.; BIROCCO AM.; SAAVEDRA SL.; LÁZARO-MARTINEZ JM.; CAMPERI S.; GLISONI RJ.; SOSNIK A.; CALABRESE GC.

CARBOHYDRATE POLYMERS

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2020 vol. 230 p. 1 - 11

0144-8617

This work describes a novel delivery system for targeting egg-derived anti-inflammatory tripeptide Ile-Arg-Trp (IRW) to endothelial cells. The nanomedicine by a simple and reproducible ionotropic gelification method that results in the efficient loading of the positively charged IRW within the dermatan sulfate/ chitosan matrix, as demonstrated by ss30 NMR spectroscopy. The incorporation of IRW results in a stable nanoparticle dispersion with a single size population of 442 ± 43 nm. Fluorescence microscopy studies demonstrate the capacity of the nanomaterial to distinguish between a quiescent and an injured endothelium through the interaction of dermatan sulfate with the CD44 receptor. Remarkably, no additional surface functionalization is required as dermatan sulfate mediates their internalization and intracellular release of this natural anti-inflammatory tripeptide. We report on the capability of IRW-loaded complexes to modulate endothelial inflammatory response. This simple, scalable, and versatilenanotechnology platform opens new opportunities to apply in the therapy of vascular disease.