DETECTION OF NEURAL EXTRACELLULAR VESICLE PROTEINS IN BLOOD AS PREDICTIVE EARLY BIOMARKERS OF ENCEPHALOPATHY ASSOCIATED WITH HEMOLYTIC UREMIC SYNDROME (HUS)

ANA B. CELI 1 , NATALIA SZPILBARG 2 , ROMINA GLISONI 3 , ANALIA LÓPEZ DÍAZ 4 , ADRIANA CANGELOSI 5 , PATRICIA A. GEOGHEGAN 5 , ALIPIO PINTO 1 ,JORGE GOLDSTEIN

MAR DEL PLATA

Congreso; SAIC 2022; 2022

Shiga toxin (Stx) producing E. coli (STEC) is the principal etiologic agent causing HUS, characterized by hemolytic anemia, thrombocytopenia and renal failure.Neurological alterations may occur, and consequently a poor prognosis and increased mortality rate are recorded. STEC is a gram-negative bacterium, and in addition to Stx also releases LPS, involved in proinflammatory-related events which contributes significantly to the development of the disease. The detection of serum biomarkers associated with neural injury during the first days of bloody diarrhea onset, and prior to HUS signs and symptoms, could be determinant to start pharmacological strategies to prevent neural damage. The aim of this work was to determine whether neuronal tau and astrocyte GFAP proteins can be considered early serological biomarkers of encephalopathy in the context of HUS. For this purpose, NIH-Swiss male mice were intravenously injected with vehicle, LPS (800ng), Stx2 (3.5ng, 1LD 100 ) or a combination of Stx2+LPS (same previous amounts). After 1- and 2- days, blood samples were collected to detect tau protein by Elisa (Invitrogen, Viena, Austria) and extracellular vesicles (EVs) were obtained from plasma by differential centrifugation. These EVs were characterized in identity, quantity and size using western blot, Bradford and Dynamic Light Scattering (DLS) techniques. One way ANOVA and Tukey post-hoc tests were employed for statistical analysis. A significant two-fold increase of tau protein was determined after 2 days in the Stx2+LPS group (p